Hotspot

trnL-UUG-ndhB

IRb · 92793-93392 · 600 bp

Back to Cryptospora

Pi 0.00171

MarkerSeek score 62.00

Primer available yes

Label trnL-UUG-ndhB

Feature Metadata

label_name: trnL-UUG-ndhB
feature_type: igs
region: IRb
length_bp: 600
variable_sites: 1
pi: 0.00171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUG-ndhB_p1	GTCTCGCGTGTCTACCATTT	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	662-676 bp	1.0	81.5
trnL-UUG-ndhB_p2	CTCGCGTGTCTACCATTTCA	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	660-674 bp	1.0	81.5
trnL-UUG-ndhB_p3	GTCTCGCGTGTCTACCATTT	TTGCGATTGCTCAGGATACC	50.0	50.0	58.0	58.0	653-667 bp	1.0	81.4
trnL-UUG-ndhB_p4	CTCGCGTGTCTACCATTTCA	TTGCGATTGCTCAGGATACC	50.0	50.0	58.0	58.0	651-665 bp	1.0	81.4
trnL-UUG-ndhB_p5	AACCTCCACGCTTTTTAGCA	ACCCGATTATTGCGATTGCT	45.0	45.0	58.0	58.0	693-707 bp	1.0	81.3