Hotspot

psbT-psbN

LSC · 72955-73019 · 65 bp

Back to Cryptospora

Pi 0.0308

MarkerSeek score 68.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GGAACCACCTACAATTTCAACT	GATCCATTCGAGGAACACGG	40.9	55.0	56.5	58.4	123 bp	1.0	50.1
psbT-psbN_p2	GGAACCACCTACAATTTCAACT	GGCAACCCTCTCAACAACTA	40.9	50.0	56.5	57.4	146 bp	1.0	48.9
psbT-psbN_p3	GGAACCACCTACAATTTCAACT	AGATCCATTCGAGGAACACG	40.9	50.0	56.5	57.4	124 bp	1.0	48.8
psbT-psbN_p4	GGAACCACCTACAATTTCAAC	GATCCATTCGAGGAACACGG	42.9	55.0	55.0	58.4	123 bp	1.0	46.2
psbT-psbN_p5	GGAACCACCTACAATTTCAAC	GGCAACCCTCTCAACAACTA	42.9	50.0	55.0	57.4	146 bp	1.0	44.9