Hotspot

psbE-petL

LSC · 63130-64395 · 1266 bp

Back to Cryptospora

Pi 0.00952

MarkerSeek score 64.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 12
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1417-1420 bp	1.0	66.7
psbE-petL_p2	CAAATAACCAGCCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.1	56.3	1420-1423 bp	1.0	66.7
psbE-petL_p3	AAATAACCAGCCCGCAATGA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1419-1422 bp	1.0	66.1
psbE-petL_p4	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1418-1421 bp	1.0	66.1
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1335-1338 bp	1.0	66.1