Hotspot

trnT-ACC-psbD

LSC · 30903-32134 · 1232 bp

Back to Cryptospora

Pi 0.00652

MarkerSeek score 62.90

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 8
pi: 0.00652
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	TCAGGAATTAAACCGAGGCG	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.0	57.9	1415-1422 bp	1.0	79.5
trnT-ACC-psbD_p2	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	1381-1388 bp	1.0	79.2
trnT-ACC-psbD_p3	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1384-1391 bp	1.0	79.0
trnT-ACC-psbD_p4	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	1358-1365 bp	1.0	77.4
trnT-ACC-psbD_p5	GGCCCTTTTAACTCAGTGGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	1396-1403 bp	1.0	77.4