Hotspot

rpl22

Genome · 15004-15384 · 381 bp

Back to Cryptomeria

Pi 0.00262

MarkerSeek score 42.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: Genome
length_bp: 381
variable_sites: 1
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TAGCTCGAAGGGTCTGTAGG	TTCCAGGGACATGCGAAAAA	55.0	45.0	57.9	58.0	1226 bp	1.0	79.9
rpl22_p2	TAGCTCGAAGGGTCTGTAGG	TTTCCAGGGACATGCGAAAA	55.0	45.0	57.9	58.0	1227 bp	1.0	79.9
rpl22_p3	TAGCTCGAAGGGTCTGTAGG	TTTTCCAGGGACATGCGAAA	55.0	45.0	57.9	58.0	1228 bp	1.0	79.9
rpl22_p4	TAGCTCGAAGGGTCTGTAGG	TCAAAACGTTGGCTGGGTAA	55.0	45.0	57.9	57.9	1718 bp	1.0	79.5
rpl22_p5	TAGCTCGAAGGGTCTGTAGG	ATAGTAACCTGGTCCCGGAG	55.0	55.0	57.9	57.9	1373 bp	1.0	79.3