Hotspot

ndhF-CrjaCt024

Genome · 98392-99193 · 802 bp

Back to Cryptomeria

Pi 0.00374
MarkerSeek score 60.90
Primer available yes
Label ndhF-CrjaCt024

Feature Metadata

label_name
ndhF-CrjaCt024
feature_type
igs
region
Genome
length_bp
802
variable_sites
3
pi
0.00374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-CrjaCt024_p1 ATGAGATGCTTAGGAGGGGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 58.3 57.7 983 bp 1.0 75.7
ndhF-CrjaCt024_p2 GCTTAGGAGGGGGACGTATA TTCCTCAGTAGCTCAGTGGT 55.0 50.0 57.7 57.7 976 bp 1.0 75.4
ndhF-CrjaCt024_p3 ATGAGATGCTTAGGAGGGGG CCGATTGGTCGTAGGTTCAA 55.0 50.0 58.3 57.6 945 bp 1.0 75.2
ndhF-CrjaCt024_p4 GCTTAGGAGGGGGACGTATA CCGATTGGTCGTAGGTTCAA 55.0 50.0 57.7 57.6 938 bp 1.0 74.9
ndhF-CrjaCt024_p5 AGGAGAGGGAATGAGATGCT TTCCTCAGTAGCTCAGTGGT 50.0 50.0 57.5 57.7 993 bp 1.0 73.8