Hotspot

CrjaCt019-psaM

Genome · 83433-83969 · 537 bp

Back to Cryptomeria

Pi 0
MarkerSeek score 38.70
Primer available yes
Label CrjaCt019-psaM

Feature Metadata

label_name
CrjaCt019-psaM
feature_type
igs
region
Genome
length_bp
537
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
CrjaCt019-psaM_p1 ACGAGAGTTGAAAGATCCTTCA AGCAGCTAAAAGGGCTATCA 40.9 45.0 57.1 56.6 658 bp 1.0 45.8
CrjaCt019-psaM_p2 ACGAGAGTTGAAAGATCCTTCA AAGCAGCTAAAAGGGCTATCA 40.9 42.9 57.1 57.3 659 bp 1.0 43.8
CrjaCt019-psaM_p3 ACGAGAGTTGAAAGATCCTTCA AGCAGCTAAAAGGGCTATCAA 40.9 42.9 57.1 57.3 658 bp 1.0 43.8
CrjaCt019-psaM_p4 ACGAGAGTTGAAAGATCCTTCAA AGCAGCTAAAAGGGCTATCA 39.1 45.0 57.8 56.6 658 bp 1.0 43.0
CrjaCt019-psaM_p5 ACGAGAGTTGAAAGATCCTTCAA AGCAGCTAAAAGGGCTATCAA 39.1 42.9 57.8 57.3 658 bp 1.0 41.1