Hotspot

atpI-atpH

Genome · 78315-78891 · 577 bp

Back to Cryptomeria

Pi 0.00173

MarkerSeek score 59.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 577
variable_sites: 1
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGAATCCATGGAGGGTCATC	CCTACGGATAATCCAGCAGC	55.0	55.0	57.8	57.9	655 bp	1.0	78.0
atpI-atpH_p2	CGAATCCATGGAGGGTCATC	GCCTACGGATAATCCAGCAG	55.0	55.0	57.8	57.9	656 bp	1.0	78.0
atpI-atpH_p3	CGAATCCATGGAGGGTCATC	AACGGAAGCAGCAGAAATCA	55.0	45.0	57.8	58.1	632 bp	1.0	77.9
atpI-atpH_p4	CGAATCCATGGAGGGTCATC	ACGGAAGCAGCAGAAATCAA	55.0	45.0	57.8	58.1	631 bp	1.0	77.9
atpI-atpH_p5	CGAATCCATGGAGGGTCATC	GCCAGGTCCAATAGAAGCAA	55.0	50.0	57.8	57.9	677 bp	1.0	77.7