Hotspot

ndhJ-chlN

Genome · 36648-37175 · 528 bp

Back to Cryptomeria

Pi 0.00189

MarkerSeek score 58.30

Primer available yes

Label ndhJ-chlN

Feature Metadata

label_name: ndhJ-chlN
feature_type: igs
region: Genome
length_bp: 528
variable_sites: 1
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-chlN_p1	GTTGGATTGGGTGGCCTTTA	GACTTGGGTCCAACCACTTT	50.0	50.0	58.1	57.9	632 bp	1.0	79.0
ndhJ-chlN_p2	TTGGATTGGGTGGCCTTTAC	GACTTGGGTCCAACCACTTT	50.0	50.0	58.1	57.9	631 bp	1.0	79.0
ndhJ-chlN_p3	GTTGGATTGGGTGGCCTTTA	CCTCTACGGCGTCAGAAAAA	50.0	50.0	58.1	57.9	659 bp	1.0	79.0
ndhJ-chlN_p4	TTGGATTGGGTGGCCTTTAC	CCTCTACGGCGTCAGAAAAA	50.0	50.0	58.1	57.9	658 bp	1.0	79.0
ndhJ-chlN_p5	TTGGATTGGGTGGCCTTTAC	ACTTGGGTCCAACCACTTTG	50.0	50.0	58.1	58.2	630 bp	1.0	77.8