Hotspot

accD-rbcL

Genome · 27981-28749 · 769 bp

Back to Cryptomeria

Pi 0.0013

MarkerSeek score 59.50

Primer available yes

Label accD-rbcL

Feature Metadata

label_name: accD-rbcL
feature_type: igs
region: Genome
length_bp: 769
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rbcL_p1	AAGGGTCTCACAATTCTCGC	GGTAATCAAGTGATCCGCGA	50.0	50.0	57.9	57.8	997 bp	1.0	77.5
accD-rbcL_p2	AAGGGTCTCACAATTCTCGC	AGGTAATCAAGTGATCCGCG	50.0	50.0	57.9	57.8	998 bp	1.0	77.5
accD-rbcL_p3	TTTCTACGGGGGTTGTTTCG	GGTAATCAAGTGATCCGCGA	50.0	50.0	58.1	57.8	935 bp	1.0	77.4
accD-rbcL_p4	TTTCTACGGGGGTTGTTTCG	AGGTAATCAAGTGATCCGCG	50.0	50.0	58.1	57.8	936 bp	1.0	77.3
accD-rbcL_p5	GGTCTCACAATTCTCGCACA	GGTAATCAAGTGATCCGCGA	50.0	50.0	58.2	57.8	994 bp	1.0	76.7