Hotspot

rps16

LSC · 22102-24669 · 2568 bp

Back to Cryptocoryne

Pi 0.0354

MarkerSeek score 54.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 2568
variable_sites: 50
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACA	ATGACCGAGTTCTATGCCCA	45.0	50.0	58.2	58.5	2263-2973 bp	1.0	79.9
rps16_p2	ACTTGTGTTGGATTGGCACA	TGACCGAGTTCTATGCCCAT	45.0	50.0	58.2	58.5	2262-2972 bp	1.0	79.9
rps16_p3	ACTTGTGTTGGATTGGCACA	AATGACCGAGTTCTATGCCC	45.0	50.0	58.2	57.4	2264-2974 bp	1.0	79.0
rps16_p4	AGACTTGTGTTGGATTGGCA	ATGACCGAGTTCTATGCCCA	45.0	50.0	57.6	58.5	2265-2975 bp	1.0	78.8
rps16_p5	AGACTTGTGTTGGATTGGCA	TGACCGAGTTCTATGCCCAT	45.0	50.0	57.6	58.5	2264-2974 bp	1.0	78.8