Hotspot

ycf1-rps15

SSC · 149162-150395 · 1234 bp

Back to Cryptocoryne

Pi 0.0525

MarkerSeek score 85.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 1234
variable_sites: 48
pi: 0.0525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ACAGATGTATCCACAGCACA	TCGACAGACCCTTTGTTTTCT	45.0	42.9	56.5	57.4	1296-1339 bp	1.0	62.9
ycf1-rps15_p2	ACAGATGTATCCACAGCACA	ACTTGAAATTCGACAGACCCT	45.0	42.9	56.5	57.2	1305-1348 bp	1.0	61.1
ycf1-rps15_p3	ACAGATGTATCCACAGCACAA	TCGACAGACCCTTTGTTTTCT	42.9	42.9	57.3	57.4	1296-1339 bp	1.0	60.9
ycf1-rps15_p4	ACAGATGTATCCACAGCACAA	ACTTGAAATTCGACAGACCCT	42.9	42.9	57.3	57.2	1305-1348 bp	1.0	59.1
ycf1-rps15_p5	ACAGATGTATCCACAGCACA	TCGACAGACCCTTTGTTTTC	45.0	45.0	56.5	55.9	1296-1339 bp	1.0	58.6