Hotspot

petA-psbJ

LSC · 93760-95511 · 1752 bp

Back to Cryptocoryne

Pi 0.0942

MarkerSeek score 79.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1752
variable_sites: 146
pi: 0.0942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCTGACGGAAGCAAGAGTA	GGGGTCAATTTCAGCAATGC	50.0	50.0	58.1	58.0	2418-2555 bp	1.0	88.1
petA-psbJ_p2	TCGTATTTCCCCCGAGATGA	GGGGTCAATTTCAGCAATGC	50.0	50.0	57.9	58.0	2637-2774 bp	1.0	87.8
petA-psbJ_p3	TGATCGTATTTCCCCCGAGA	GGGGTCAATTTCAGCAATGC	50.0	50.0	57.9	58.0	2640-2777 bp	1.0	87.8
petA-psbJ_p4	TCGTATTTCCCCCGAGATGA	GAGAACCGACAAGGGATTCC	50.0	55.0	57.9	58.0	2811-2948 bp	1.0	87.5
petA-psbJ_p5	TGATCGTATTTCCCCCGAGA	GAGAACCGACAAGGGATTCC	50.0	55.0	57.9	58.0	2814-2951 bp	1.0	87.5