Hotspot

psbZ-trnG-GCC

LSC · 59664-60504 · 841 bp

Back to Cryptocoryne

Pi 0.0683

MarkerSeek score 75.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 48
pi: 0.0683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAAGACCGGAGCTATCAACC	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.1	58.0	1971-2003 bp	1.0	85.7
psbZ-trnG-GCC_p2	TTCTATCCCACCTAGCCGAG	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.0	58.0	2139 bp	0.5	60.0
psbZ-trnG-GCC_p3	TTCTATCCCACCTAGCCGAG	GCAACAAGGTCCAGTTGGTA	55.0	50.0	58.0	58.0	2037 bp	0.5	59.8
psbZ-trnG-GCC_p4	TTCTATCCCACCTAGCCGAG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	1820 bp	0.5	59.7
psbZ-trnG-GCC_p5	TTCTATCCCACCTAGCCGAG	GGCCCCTCTTTACCATTCTG	55.0	55.0	58.0	57.9	1959 bp	0.5	59.5