Hotspot

psbC-trnS-UGA

LSC · 58897-59033 · 137 bp

Back to Cryptocoryne

Pi 0.0949

MarkerSeek score 81.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 13
pi: 0.0949
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	244 bp	1.0	82.5
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	190 bp	1.0	82.5
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	255 bp	1.0	81.8
psbC-trnS-UGA_p4	TTCCATGACCCCTCTTAGCT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.7	58.1	236 bp	1.0	81.8
psbC-trnS-UGA_p5	TTGTGGGTCATTTGTGGCAT	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.3	58.1	324 bp	1.0	80.5