Hotspot

petN-psbM

LSC · 51351-52890 · 1540 bp

Back to Cryptocoryne

Pi 0.0431

MarkerSeek score 82.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1540
variable_sites: 62
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTAAGTCTCGCTTGGGCTAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1700-1937 bp	1.0	74.9
petN-psbM_p2	CTCGTAGTATGGGGGAGGAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.1	57.6	1649-1886 bp	1.0	74.8
petN-psbM_p3	AGTAAGTCTCGCTTGGGCTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1701-1938 bp	1.0	74.3
petN-psbM_p4	TAAGTCTCGCTTGGGCTACT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1699-1936 bp	1.0	74.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1702-1939 bp	1.0	74.3