Hotspot

rpoB-trnC-GCA

LSC · 48734-50144 · 1411 bp

Back to Cryptocoryne

Pi 0.0297

MarkerSeek score 69.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1411
variable_sites: 35
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCCGGAG	TTGTTAATCAGGCGACACCC	55.0	50.0	58.0	58.2	1299-1514 bp	1.0	84.3
rpoB-trnC-GCA_p2	CTCATTTTCATCCCGGAGCA	TTGTTAATCAGGCGACACCC	50.0	50.0	58.0	58.2	1297-1512 bp	1.0	84.2
rpoB-trnC-GCA_p3	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1288-1503 bp	1.0	82.0
rpoB-trnC-GCA_p4	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1286-1501 bp	1.0	81.9
rpoB-trnC-GCA_p5	CCCTCATTTTCATCCCGGAG	TAATCAGGCGACACCCAGAT	55.0	50.0	58.0	58.5	1295-1510 bp	1.0	81.7