Hotspot

atpF-atpH

LSC · 34131-34672 · 542 bp

Back to Cryptocoryne

Pi 0.0481

MarkerSeek score 74.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 25
pi: 0.0481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAATGGCCAGAGGTTCAAGG	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.1	58.0	620-634 bp	1.0	85.6
atpF-atpH_p2	AAATGGCCAGAGGTTCAAGG	GGTATTGCAAGACAGCCAGA	50.0	50.0	58.1	57.9	711-725 bp	1.0	84.1
atpF-atpH_p3	TACACAACTCGCACACACTC	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.2	58.0	731-745 bp	1.0	84.0
atpF-atpH_p4	AAATGGCCAGAGGTTCAAGG	TGATTTCTGCCGCTTCTGTT	50.0	45.0	58.1	58.1	812-826 bp	1.0	83.5
atpF-atpH_p5	TTGTGCCTGGACTTCAAACA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.9	58.0	976-990 bp	1.0	83.0