Hotspot

trnS-GCU-trnG-UCC

LSC · 26688-29585 · 2898 bp

Back to Cryptocoryne

Pi 0.0365

MarkerSeek score 54.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 2898
variable_sites: 42
pi: 0.0365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGTCGCTTTAGTCCA	GGACGAATCACACTTTTACCAC	50.0	45.5	58.0	57.6	1238-2969 bp	1.0	66.4
trnS-GCU-trnG-UCC_p2	AATCCGTCGCTTTAGTCCAC	GGACGAATCACACTTTTACCAC	50.0	45.5	58.0	57.6	1237-2968 bp	1.0	66.4
trnS-GCU-trnG-UCC_p3	AGCAATCCGTCGCTTTAGTC	GGACGAATCACACTTTTACCAC	50.0	45.5	58.4	57.6	1240-2971 bp	1.0	63.5
trnS-GCU-trnG-UCC_p4	CGTCGCTTTAGTCCACTCAG	GGACGAATCACACTTTTACCAC	55.0	45.5	58.4	57.6	1233-2964 bp	1.0	63.4
trnS-GCU-trnG-UCC_p5	CAATCCGTCGCTTTAGTCCA	GGACGAATCACACTTTTACCA	50.0	42.9	58.0	56.2	1238-2969 bp	1.0	63.1