Hotspot

psbA-trnK-UUU

LSC · 18522-18911 · 390 bp

Back to Cryptocoryne

Pi 0.0886

MarkerSeek score 84.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 31
pi: 0.0886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAGACGGTTTTCGGTGCTAG	CAACTAGTTCCGGGTTCGAG	50.0	55.0	58.2	58.0	471-510 bp	1.0	87.3
psbA-trnK-UUU_p2	AAAGACGGTTTTCGGTGCTA	CAACTAGTTCCGGGTTCGAG	45.0	55.0	57.8	58.0	472-511 bp	1.0	87.0
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	CAACTAGTTCCGGGTTCGAG	45.0	55.0	57.8	58.0	473-512 bp	1.0	87.0
psbA-trnK-UUU_p4	GTAAAGACGGTTTTCGGTGC	CAACTAGTTCCGGGTTCGAG	50.0	55.0	57.7	58.0	474-513 bp	1.0	86.5
psbA-trnK-UUU_p5	GACGGTTTTCGGTGCTAGTA	CAACTAGTTCCGGGTTCGAG	50.0	55.0	57.6	58.0	469-508 bp	1.0	86.2