Hotspot

trnH-GUG-psbA

LSC · 16746-17459 · 714 bp

Back to Cryptocoryne

Pi 0.0757

MarkerSeek score 73.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 39
pi: 0.0757
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GGGTAGAGCCGGATCTAAGT	TAGTTGACAGTCAGGGTCGT	55.0	50.0	58.0	58.0	1183 bp	0.5	59.9
trnH-GUG-psbA_p2	TGGGTAGAGCCGGATCTAAG	TAGTTGACAGTCAGGGTCGT	55.0	50.0	58.0	58.0	1184 bp	0.5	59.9
trnH-GUG-psbA_p3	GGGTAGAGCCGGATCTAAGT	GCTGCTGTTGAAATTCCGTC	55.0	50.0	58.0	58.0	1070 bp	0.5	59.7
trnH-GUG-psbA_p4	TGGGTAGAGCCGGATCTAAG	GCTGCTGTTGAAATTCCGTC	55.0	50.0	58.0	58.0	1071 bp	0.5	59.7
trnH-GUG-psbA_p5	GGGTAGAGCCGGATCTAAGT	CATCGTAGCTGCTCATGGTT	55.0	50.0	58.0	58.1	1359 bp	0.5	59.5