Hotspot

rps2

LSC · 16022-16732 · 711 bp

Back to Cryptocarya

Pi 0.00113
MarkerSeek score 34.00
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
2
pi
0.00113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TTTCTCAACTCGCCTGCTTT GTTTTCAAGAAACCGCTCGG 45.0 50.0 58.0 58.0 1342-1353 bp 1.0 80.9
rps2_p2 TTTCTCAACTCGCCTGCTTT CGCTCGGGTTTTAGCAAAAG 45.0 50.0 58.0 58.0 1329-1340 bp 1.0 80.9
rps2_p3 CTAACTCCCCATGCGGTAAC GTTTTCAAGAAACCGCTCGG 55.0 50.0 58.1 58.0 1787-1798 bp 1.0 80.4
rps2_p4 CTAACTCCCCATGCGGTAAC CGCTCGGGTTTTAGCAAAAG 55.0 50.0 58.1 58.0 1774-1785 bp 1.0 80.4
rps2_p5 TATATCATTCGTGGGCGCAG GTTTTCAAGAAACCGCTCGG 50.0 50.0 57.9 58.0 1807-1818 bp 1.0 80.2