Hotspot

rbcL

LSC · 57755-59182 · 1428 bp

Back to Cryptocarya

Pi 0.00476

MarkerSeek score 54.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 16
pi: 0.00476
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1495 bp	1.0	72.1
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1496 bp	1.0	72.1
rbcL_p3	TGAGTTGTAGGGAGGGACTT	CAATTAGGGGAACGAACGGA	50.0	50.0	57.3	57.6	1478 bp	1.0	72.0
rbcL_p4	TGAGTTGTAGGGAGGGACTT	TGCAATTAGGGGAACGAACG	50.0	50.0	57.3	58.6	1480 bp	1.0	70.7
rbcL_p5	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1498 bp	1.0	70.3