Hotspot

clpP

LSC · 73112-75150 · 2039 bp

Back to Cryptocarya

Pi 0.00275

MarkerSeek score 52.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2039
variable_sites: 12
pi: 0.00275
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGTTCATTCAAATTTCGAAATGG	ACGTTTCCACATCAAAGTGA	33.3	40.0	57.0	55.5	2081-2133 bp	1.0	42.2
clpP_p2	TGGTTCATTCAAATTTCGAAATGG	ACGTTTCCACATCAAAGTGAA	33.3	38.1	57.0	56.3	2081-2133 bp	1.0	42.2
clpP_p3	TGGTTCATTCAAATTTCGAAATGGA	ACGTTTCCACATCAAAGTGA	32.0	40.0	58.2	55.5	2081-2133 bp	1.0	42.2
clpP_p4	GGTTCATTCAAATTTCGAAATGGA	ACGTTTCCACATCAAAGTGA	33.3	40.0	56.7	55.5	2080-2132 bp	1.0	42.2
clpP_p5	TGGTTCATTCAAATTTCGAAATGGA	ACGTTTCCACATCAAAGTGAA	32.0	38.1	58.2	56.3	2081-2133 bp	1.0	42.2