Hotspot

rpl32-trnL-UAG

SSC · 115709-116772 · 1064 bp

Back to Cryptocarya

Pi 0.00584
MarkerSeek score 48.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1064
variable_sites
14
pi
0.00584
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1168-1179 bp 1.0 78.9
rpl32-trnL-UAG_p2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 1161-1172 bp 1.0 78.4
rpl32-trnL-UAG_p3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.7 57.8 1167-1178 bp 1.0 78.3
rpl32-trnL-UAG_p4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 1166-1177 bp 1.0 78.3
rpl32-trnL-UAG_p5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.7 58.3 1162-1173 bp 1.0 77.7