Hotspot

ndhF-rpl32

SSC · 114370-115552 · 1183 bp

Back to Cryptocarya

Pi 0.00441

MarkerSeek score 46.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1183
variable_sites: 11
pi: 0.00441
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGAACAAACAGAAGTCCCA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1273-1296 bp	1.0	79.4
ndhF-rpl32_p2	TCGGAACAAACAGAAGTCCC	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.8	57.7	1274-1297 bp	1.0	77.7
ndhF-rpl32_p3	CGGTCGGAACAAACAGAAGT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.4	57.7	1277-1300 bp	1.0	76.3
ndhF-rpl32_p4	CGGAACAAACAGAAGTCCCA	TACGTTTCTTTGGAACCGCC	50.0	50.0	58.0	58.8	1272-1295 bp	1.0	75.6
ndhF-rpl32_p5	TGGAAGTGGAGCGAAAGGTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.7	57.7	1234-1257 bp	1.0	74.5