Hotspot

trnS-GGA-rps4

LSC · 47007-47294 · 288 bp

Back to Cryptocarya

Pi 0.00709

MarkerSeek score 62.50

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 5
pi: 0.00709
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	CGGTTTGAAAATCAATGAGTTGC	55.0	39.1	58.4	57.7	422-435 bp	1.0	54.3
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	TCAATGAGTTGCTAGTCGTAGA	55.0	40.9	58.4	56.7	411-424 bp	1.0	54.0
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	ATCAATGAGTTGCTAGTCGT	55.0	40.0	58.4	54.5	412-425 bp	1.0	52.4
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	TGACTTTTCACTCATCCCAA	55.0	40.0	58.4	54.5	483-496 bp	1.0	51.8
trnS-GGA-rps4_p5	ATGTAGGAGAGATGGCCGAG	TTGACTTTTCACTCATCCCA	55.0	40.0	58.4	54.5	484-497 bp	1.0	51.8