Hotspot

atpH-atpI

LSC · 14147-15060 · 914 bp

Back to Cryptocarya

Pi 0.0049

MarkerSeek score 67.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 8
pi: 0.0049
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.1	57.9	974-1052 bp	1.0	82.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1032-1110 bp	1.0	82.7
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	CCCGTCATGTTCCTTGGATT	55.0	50.0	58.1	57.9	1061-1139 bp	1.0	82.5
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	CCCGTCATGTTCCTTGGATT	55.0	50.0	57.9	57.9	1062-1140 bp	1.0	82.5
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	992-1070 bp	1.0	82.4