Hotspot

trnK-UUU-rps16

LSC · 4372-5180 · 809 bp

Back to Cryptocarya

Pi 0.00515

MarkerSeek score 65.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 9
pi: 0.00515
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCAAAGAAGGCGGAGGTG	50.0	50.0	58.2	58.4	875-903 bp	1.0	78.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCAAAGAAGGCGGAGGTG	50.0	50.0	58.2	58.4	874-902 bp	1.0	78.6
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	AGAAGGCGGAGGTGTTTAAG	50.0	50.0	58.2	57.5	869-897 bp	1.0	78.0
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGAAGGCGGAGGTGTTTAAG	50.0	50.0	58.2	57.5	868-896 bp	1.0	78.0
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	TTTCAAAGAAGGCGGAGGTG	50.0	50.0	58.3	58.4	884-912 bp	1.0	77.7