Hotspot

ycf1

SSC · 124265-129643 · 5379 bp

Back to Crucihimalaya

Pi 0.0131

MarkerSeek score 47.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 138
pi: 0.0131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACAGCTGTTCGATGCAAA	GATTGGAAACGCGAAATGCA	45.0	45.0	58.1	58.0	822 bp	0.75	70.0
ycf1_p2	AGACAGCTGTTCGATGCAAA	TTCAATTTCCCGAGTGGTCC	45.0	50.0	58.1	57.8	854 bp	0.75	68.6
ycf1_p3	AGACAGCTGTTCGATGCAAA	AGATTGGAAACGCGAAATGC	45.0	45.0	58.1	57.7	823 bp	0.75	68.1
ycf1_p4	AGACAGCTGTTCGATGCAAA	AGTTTGGGGAATGGAAACCG	45.0	50.0	58.1	58.4	604 bp	0.75	67.4
ycf1_p5	AGACAGCTGTTCGATGCAAA	TTGGGGAATGGAAACCGAAC	45.0	50.0	58.1	58.4	601 bp	0.75	67.4