Hotspot

ndhA

SSC · 120232-122383 · 2152 bp

Back to Crucihimalaya

Pi 0.0101

MarkerSeek score 65.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 43
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2306-2317 bp	1.0	56.3
ndhA_p2	ACCCGGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2307-2318 bp	1.0	56.3
ndhA_p3	CCCGGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	50.0	45.0	56.8	55.9	2307-2318 bp	1.0	55.9
ndhA_p4	ACCCGGTTATCATAGGAAGC	ATGACGATACTCGGTAGCAT	50.0	45.0	56.8	55.9	2308-2319 bp	1.0	55.8
ndhA_p5	AACCCGGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	47.6	45.5	57.5	58.0	2308-2319 bp	1.0	54.2