Hotspot

rpl32-trnL-CUA

SSC · 113845-114638 · 794 bp

Back to Crucihimalaya

Pi 0.0347

MarkerSeek score 72.20

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 794
variable_sites: 50
pi: 0.0347
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	CTACAGGTAATTCAAAAAGTTTTT	TCCTAAGAGCAGCGTGTCTA	25.0	50.0	52.4	57.9	877-903 bp	1.0	45.7
rpl32-trnL-CUA_p2	CTACAGGTAATTCAAAAAGTTTTT	AGCAGCGTGTCTACCAATTT	25.0	45.0	52.4	57.8	870-896 bp	1.0	45.7
rpl32-trnL-CUA_p3	CTACAGGTAATTCAAAAAGTTTTT	CCTAAGAGCAGCGTGTCTAC	25.0	55.0	52.4	57.8	876-902 bp	1.0	45.7
rpl32-trnL-CUA_p4	CTACAGGTAATTCAAAAAGTTTTT	CTAAGAGCAGCGTGTCTACC	25.0	55.0	52.4	57.8	875-901 bp	1.0	45.7
rpl32-trnL-CUA_p5	CTACAGGTAATTCAAAAAGTTTTT	GAGCAGCGTGTCTACCAATT	25.0	50.0	52.4	58.3	871-897 bp	1.0	45.7