Hotspot

accD-psaI

LSC · 58289-58960 · 672 bp

Back to Crucihimalaya

Pi 0.0191

MarkerSeek score 65.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 25
pi: 0.0191
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTCCATGC	TGCAATTGCCGGAAAGACTA	45.0	45.0	54.5	57.8	761-850 bp	1.0	55.4
accD-psaI_p2	GAGTTATTTCAGCTCCATGC	CCGGAAAGACTAGGCCTACT	45.0	55.0	54.5	58.2	753-842 bp	1.0	55.3
accD-psaI_p3	GAGTTATTTCAGCTCCATGC	CATTGCAATTGCCGGAAAGA	45.0	45.0	54.5	57.6	764-853 bp	1.0	54.0
accD-psaI_p4	GAGTTATTTCAGCTCCATGC	ATTGCAATTGCCGGAAAGAC	45.0	45.0	54.5	57.6	763-852 bp	1.0	54.0
accD-psaI_p5	GAGTTATTTCAGCTCCATGC	CCATTGCAATTGCCGGAAAG	45.0	50.0	54.5	58.6	765-854 bp	1.0	51.9