Hotspot

ndhC-trnV-GUA

LSC · 50056-50946 · 891 bp

Back to Crucihimalaya

Pi 0.015
MarkerSeek score 66.50
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
891
variable_sites
25
pi
0.015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 936-984 bp 1.0 71.6
ndhC-trnV-GUA_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 935-983 bp 1.0 69.6
ndhC-trnV-GUA_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 936-984 bp 1.0 69.5
ndhC-trnV-GUA_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 964-1012 bp 1.0 68.6
ndhC-trnV-GUA_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 964-1012 bp 1.0 68.3