Hotspot

ndhK-ndhC

LSC · 49628-49692 · 65 bp

Back to Crucihimalaya

Pi 0.0615

MarkerSeek score 80.80

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 7
pi: 0.0615
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GTTGTTCGATCAAGTACGGGA	CATGGCGAAAAGGAGCATTG	47.6	50.0	58.0	58.1	133-162 bp	1.0	76.0
ndhK-ndhC_p2	TGTTGTTCGATCAAGTACGGG	CATGGCGAAAAGGAGCATTG	47.6	50.0	58.3	58.1	134-163 bp	1.0	73.8
ndhK-ndhC_p3	TGTTCGATCAAGTACGGGAA	CATGGCGAAAAGGAGCATTG	45.0	50.0	56.6	58.1	131-160 bp	1.0	73.3
ndhK-ndhC_p4	TTGTTCGATCAAGTACGGGA	CATGGCGAAAAGGAGCATTG	45.0	50.0	56.6	58.1	132-161 bp	1.0	73.2
ndhK-ndhC_p5	GTTGTTCGATCAAGTACGGG	CATGGCGAAAAGGAGCATTG	50.0	50.0	56.6	58.1	133-162 bp	1.0	72.9