Hotspot

trnT-ACA-trnL-UUA

LSC · 45878-46643 · 766 bp

Back to Crucihimalaya

Pi 0.0205

MarkerSeek score 71.60

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 31
pi: 0.0205
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	786-843 bp	1.0	84.7
trnT-ACA-trnL-UUA_p2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	837-894 bp	1.0	84.3
trnT-ACA-trnL-UUA_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	789-846 bp	1.0	84.0
trnT-ACA-trnL-UUA_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	818-875 bp	1.0	83.8
trnT-ACA-trnL-UUA_p5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	840-897 bp	1.0	83.6