Hotspot

psaA-ycf3

LSC · 41465-42171 · 707 bp

Back to Crucihimalaya

Pi 0.0229

MarkerSeek score 61.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 31
pi: 0.0229
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ATGCCACTCAGCCAAAGAAA	ACAGGCCATTCAACAAGGAG	45.0	50.0	58.0	58.1	1114-1118 bp	1.0	82.5
psaA-ycf3_p2	ATGCCACTCAGCCAAAGAAA	GGAGAACAGGCCATTCAACA	45.0	50.0	58.0	58.1	1119-1123 bp	1.0	82.5
psaA-ycf3_p3	ATGCCACTCAGCCAAAGAAA	GTGGAGAACAGGCCATTCAA	45.0	50.0	58.0	58.1	1121-1125 bp	1.0	82.5
psaA-ycf3_p4	ATGCCACTCAGCCAAAGAAA	TGGAGAACAGGCCATTCAAC	45.0	50.0	58.0	58.1	1120-1124 bp	1.0	82.5
psaA-ycf3_p5	GATCACTCAGCCATGCTTCA	ACAGGCCATTCAACAAGGAG	50.0	50.0	58.0	58.1	1164-1168 bp	1.0	82.1