Hotspot

trnE-GAA-trnT-ACC

LSC · 30384-31059 · 676 bp

Back to Crucihimalaya

Pi 0.0137

MarkerSeek score 64.60

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 18
pi: 0.0137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	774-938 bp	1.0	83.6
trnE-GAA-trnT-ACC_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	753-917 bp	1.0	83.5
trnE-GAA-trnT-ACC_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	753-917 bp	1.0	83.2
trnE-GAA-trnT-ACC_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	732-896 bp	1.0	83.1
trnE-GAA-trnT-ACC_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	750-914 bp	1.0	83.0