Hotspot

trnK-AAA-rps16

LSC · 4310-4910 · 601 bp

Back to Crucihimalaya

Pi 0.02
MarkerSeek score 65.20
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
601
variable_sites
24
pi
0.02
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 963-1037 bp 1.0 83.8
trnK-AAA-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 964-1038 bp 1.0 83.8
trnK-AAA-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 959-1033 bp 1.0 83.4
trnK-AAA-rps16_p4 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 960-1034 bp 1.0 83.4
trnK-AAA-rps16_p5 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 960-1034 bp 1.0 83.4