Hotspot

rpl32-trnL-UAG

SSC · 114162-114792 · 631 bp

Back to Crowea

Pi 0.0074
MarkerSeek score 63.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
631
variable_sites
7
pi
0.0074
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAAGGGGAGATTGGGCAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 772-775 bp 1.0 80.2
rpl32-trnL-UAG_p2 AAAAAGGGGAGATTGGGCAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 773-776 bp 1.0 80.2
rpl32-trnL-UAG_p3 GCGTTGAAGGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 753-756 bp 1.0 79.4
rpl32-trnL-UAG_p4 GCGTTGAAGGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 754-757 bp 1.0 79.3
rpl32-trnL-UAG_p5 AAAAAGGGGAGATTGGGCAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 782-785 bp 1.0 79.3