Hotspot

rps3-rpl22

LSC · 84034-84400 · 367 bp

Back to Crowea

Pi 0.0056
MarkerSeek score 49.80
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
367
variable_sites
2
pi
0.0056
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.65
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 CAAGTCGGAAACCAAGTGGA GTTCTTACGAGGAAGCCCTT 50.0 50.0 58.0 57.2 346-475 bp 1.0 78.0
rps3-rpl22_p2 CTTCTTGTAGGCCTTCGCAA GTTCTTACGAGGAAGCCCTT 50.0 50.0 58.2 57.2 415-544 bp 1.0 76.7
rps3-rpl22_p3 CAAGTCGGAAACCAAGTGGA TGATCAGATTCGTGGACGTT 50.0 45.0 58.0 57.0 363-492 bp 1.0 75.8
rps3-rpl22_p4 CAAGTCGGAAACCAAGTGGA TTGATCAGATTCGTGGACGT 50.0 45.0 58.0 57.0 364-493 bp 1.0 75.8
rps3-rpl22_p5 ATCTTCTTGTAGGCCTTCGC GTTCTTACGAGGAAGCCCTT 50.0 50.0 57.7 57.2 417-546 bp 1.0 75.7