Hotspot

psbE-petL

LSC · 65142-66513 · 1372 bp

Back to Crowea

Pi 0.00146
MarkerSeek score 60.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1372
variable_sites
3
pi
0.00146
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCTGCTAGGAGAAAACCA 50.0 45.0 57.9 56.7 1519-1528 bp 1.0 69.9
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGCTGCTAGGAGAAAACCAA 50.0 45.0 57.9 56.7 1518-1527 bp 1.0 69.9
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AAGCTGCTAGGAGAAAACCA 50.0 45.0 57.8 56.7 1442-1451 bp 1.0 69.1
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AGCTGCTAGGAGAAAACCAA 50.0 45.0 57.8 56.7 1441-1450 bp 1.0 69.1
psbE-petL_p5 ACGAATAACCAACCCGCAAT AGCTGCTAGGAGAAAACCAA 45.0 45.0 58.2 56.7 1527-1536 bp 1.0 69.0