Hotspot

petA-psbJ

LSC · 63429-64356 · 928 bp

Back to Crowea

Pi 0.00216
MarkerSeek score 60.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
928
variable_sites
3
pi
0.00216
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.0 58.1 1068-1070 bp 1.0 79.6
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.0 58.1 1069-1071 bp 1.0 78.9
petA-psbJ_p3 TGTCCAAGGCCTTTTGGTTT CCGGATTAGGTTCGTCCTTG 45.0 55.0 58.1 58.1 1058-1060 bp 1.0 78.8
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.2 58.1 1065-1067 bp 1.0 78.4
petA-psbJ_p5 TGTCCAAGGCCTTTTGGTTT TCCGGATTAGGTTCGTCCTT 45.0 50.0 58.1 58.1 1059-1061 bp 1.0 78.2