Hotspot

atpB-rbcL

LSC · 54901-55680 · 780 bp

Back to Crowea

Pi 0.00257
MarkerSeek score 60.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
780
variable_sites
3
pi
0.00257
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG GCTTTGAATCCAACACTCGC 55.0 50.0 58.0 58.0 920-922 bp 1.0 79.7
atpB-rbcL_p2 ATCCAGTACCGGACCAATGA GCTTTGAATCCAACACTCGC 50.0 50.0 58.1 58.0 919-921 bp 1.0 79.1
atpB-rbcL_p3 TCCAGTACCGGACCAATGAT GCTTTGAATCCAACACTCGC 50.0 50.0 58.1 58.0 918-920 bp 1.0 79.1
atpB-rbcL_p4 CATCCAGTACCGGACCAATG ATCTTTAACGCCGGCTTTGA 55.0 45.0 58.0 58.2 933-935 bp 1.0 78.5
atpB-rbcL_p5 CTACATCCAGTACCGGACCA GCTTTGAATCCAACACTCGC 55.0 50.0 58.2 58.0 923-925 bp 1.0 78.3