Hotspot

psaA-ycf3

LSC · 42756-43494 · 739 bp

Back to Crowea

Pi 0.00361
MarkerSeek score 62.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
739
variable_sites
4
pi
0.00361
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGGTTCTTGAGAAATGCCCC AGCGCAGAATTGGTTGAAGA 50.0 45.0 58.1 58.1 885-901 bp 1.0 79.6
psaA-ycf3_p2 TGGTTCTTGAGAAATGCCCC ATTGGTTGAAGATCACGGGG 50.0 50.0 58.1 57.9 877-893 bp 1.0 79.4
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AGCGCAGAATTGGTTGAAGA 50.0 45.0 57.9 58.1 859-875 bp 1.0 79.4
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT ATTGGTTGAAGATCACGGGG 50.0 50.0 57.9 57.9 851-867 bp 1.0 79.2
psaA-ycf3_p5 TGGTTCTTGAGAAATGCCCC TCAAGCGCAGAATTGGTTGA 50.0 45.0 58.1 58.4 888-904 bp 1.0 77.3