Hotspot

psbZ-trnG-GCC

LSC · 36817-37359 · 543 bp

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Pi 0.0184
MarkerSeek score 61.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
543
variable_sites
15
pi
0.0184
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CGGATGGTTGGTCGAGTAAC AATCGAACCCGCATCTTCTC 55.0 50.0 58.4 58.1 702 bp 1.0 78.3
psbZ-trnG-GCC_p2 CGGATGGTTGGTCGAGTAAC GAATCGAACCCGCATCTTCT 55.0 50.0 58.4 58.1 703 bp 1.0 78.3
psbZ-trnG-GCC_p3 TTTGCTTCTCCGGATGGTTG AATCGAACCCGCATCTTCTC 50.0 50.0 58.5 58.1 712 bp 1.0 77.5
psbZ-trnG-GCC_p4 TTTGCTTCTCCGGATGGTTG GAATCGAACCCGCATCTTCT 50.0 50.0 58.5 58.1 713 bp 1.0 77.5
psbZ-trnG-GCC_p5 ATTTGCTTCTCCGGATGGTT AATCGAACCCGCATCTTCTC 45.0 50.0 57.5 58.1 713 bp 1.0 77.1