Hotspot

trnD-GUC-trnY-GUA

LSC · 30791-31262 · 472 bp

Back to Crowea

Pi 0.00425
MarkerSeek score 59.20
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
472
variable_sites
3
pi
0.00425
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 ATTGGATTTGGCTCCATCGG TGGCAATATGTCTACGCTGG 50.0 50.0 58.3 57.8 601-603 bp 1.0 76.3
trnD-GUC-trnY-GUA_p2 ATTGGATTTGGCTCCATCGG GGCAATATGTCTACGCTGGT 50.0 50.0 58.3 57.8 600-602 bp 1.0 76.3
trnD-GUC-trnY-GUA_p3 ATTGGATTTGGCTCCATCGG GGGGACGGACTGTAAATTCG 50.0 55.0 58.3 58.4 622-624 bp 1.0 75.4
trnD-GUC-trnY-GUA_p4 ATTGGATTTGGCTCCATCGG GGGACGGACTGTAAATTCGT 50.0 50.0 58.3 57.6 621-623 bp 1.0 75.2
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 540-542 bp 1.0 75.0