Hotspot

rpoB-trnC-GCA

LSC · 26869-28110 · 1242 bp

Back to Crowea

Pi 0.00269
MarkerSeek score 60.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1242
variable_sites
5
pi
0.00269
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCATTTCCATCTCCGAGCAT AAAGGATTTGCAGTCCTCCG 50.0 50.0 57.7 58.2 1304-1305 bp 1.0 76.9
rpoB-trnC-GCA_p2 CCATTTCCATCTCCGAGCAT GCGACACCCAGATTTGAACT 50.0 50.0 57.7 58.5 1330-1331 bp 1.0 74.5
rpoB-trnC-GCA_p3 CCATTTCCATCTCCGAGCAT TTTGTTGATCAGGCGACACC 50.0 50.0 57.7 58.8 1342-1343 bp 1.0 72.2
rpoB-trnC-GCA_p4 TGTATCTGATTCAACCCGGG AAAGGATTTGCAGTCCTCCG 50.0 50.0 57.0 58.2 1340-1341 bp 1.0 71.0
rpoB-trnC-GCA_p5 CTGATTCAACCCGGGTATTGT AAAGGATTTGCAGTCCTCCG 47.6 50.0 58.0 58.2 1335-1336 bp 1.0 71.0