Hotspot

atpH-atpI

LSC · 13375-14547 · 1173 bp

Back to Crowea

Pi 0.00228
MarkerSeek score 60.40
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1173
variable_sites
4
pi
0.00228
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.0 57.9 1260-1264 bp 1.0 79.0
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT CGAATCCATGGAGGGACATC 45.0 55.0 58.0 57.8 1230-1234 bp 1.0 78.7
atpH-atpI_p3 TACCTTGACCAACTCCAGGT TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.9 57.9 1316-1320 bp 1.0 78.4
atpH-atpI_p4 GTCCAATAGAAGCAAGCCCA TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.9 57.9 1298-1302 bp 1.0 78.3
atpH-atpI_p5 TACCTTGACCAACTCCAGGT CGAATCCATGGAGGGACATC 50.0 55.0 57.9 57.8 1286-1290 bp 1.0 78.1