Hotspot

psbA-trnK-UUU

LSC · 1477-1740 · 264 bp

Back to Crowea

Pi 0.0126
MarkerSeek score 58.60
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
264
variable_sites
5
pi
0.0126
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 ATGTAAAGGCGGTTTTCGGT GAAAAATGCATGCACGGCTT 45.0 45.0 58.1 58.6 454-456 bp 1.0 75.5
psbA-trnK-UUU_p2 ATGTAAAGGCGGTTTTCGGT ACTCCATCCGACTAGTTCCG 45.0 55.0 58.1 58.6 395-397 bp 1.0 75.3
psbA-trnK-UUU_p3 ATGTAAAGGCGGTTTTCGGT ATGAAAAATGCATGCACGGC 45.0 45.0 58.1 58.6 456-458 bp 1.0 75.0
psbA-trnK-UUU_p4 ACCATCCAATGTAAAGGCGG GAAAAATGCATGCACGGCTT 50.0 45.0 58.2 58.6 462-464 bp 1.0 74.4
psbA-trnK-UUU_p5 CCATCCAATGTAAAGGCGGT GAAAAATGCATGCACGGCTT 50.0 45.0 58.2 58.6 461-463 bp 1.0 74.4